Examining Phosphatases Through Immunofluorescent Microscopy, Methods in Molecular Biology (2023)

Immunofluorescent microscopy allows us to see where proteins are located and how much of them are present in cells. The location of a protein in a cell can affect its function because certain interactions happen in specific areas. This article explains how to use immunofluorescent microscopy to identify phosphatases in cells. Finding out where and how much phosphatase is present can help us understand its role in diseases and discover potential substrates and binding partners.