HDR in zebrafish using CRISPR!

After an exciting week, we microinjected our zebrafish embryos with a Crispr/Cas GFP construct in the hopes of promoting homology directed repair and tagging the hsp70i gene with GFP. Although the fish didn’t lay as many eggs as we had hoped, we still managed to inject about 50 of each construct. Based on the images taken on our Nikon fluorescent microscope, we have several positive embryos, as evidenced by the bright green fluorescence. However, there are still many checks to make sure our positives are actually positives and our negatives are actually negative. But we’re hopeful that everything is moving in the right direction towards consistent HDR in zebrafish!

—Rachel