Recent Publications – Blackburn Lab

Zebrafish Screening Identifies Erlotinib as a Wnt/β-Catenin Inhibitor in T-ALL, Biomedicine and Pharmacotherapy (2024)

The Wnt/β-catenin pathway is important in cancer and stem cell biology, making it a potential target for therapy. However, using Wnt inhibitors in the clinic is difficult due to off-target effects and interactions with other pathways. In this study, we used a zebrafish model to screen 773 FDA-approved drugs to find Wnt/β-catenin inhibitors with low…

Examining Phosphatases Through Immunofluorescent Microscopy, Methods in Molecular Biology (2023)

Immunofluorescent microscopy allows us to see where proteins are located and how much of them are present in cells. The location of a protein in a cell can affect its function because certain interactions happen in specific areas. This article explains how to use immunofluorescent microscopy to identify phosphatases in cells. Finding out where and…

Phosphatase Function of PRL-3 Is Insensitive to Divalent Metals In Vitro, ACS Omega (2023)

Phosphatase of regenerating liver 3 (PRL-3) is linked to cancer metastasis and interacts with the cyclin and CBS domain divalent metal cation transport mediator (CNNM) family of proteins to manage the levels of magnesium and other metals within cells. Despite its significance in cancer, the factors that regulate PRL-3’s activity and its interactions with CNNM…

Development of PRL-3 Specific Nanobodies, PLOS One (2023)

Phosphatase of Regenerating Liver-3 (PRL-3) is associated with the progression and spread of cancer, but the limited number of research tools available to study this protein have prevented us from fully understanding of PRL-3’s role in cancer. To address this, we developed nanobodies, or single-domain antibodies derived from alpacas, that specifically target PRL-3. Our…

Review on Zebrafish as Avatar Models, International Journal of Molecular Sciences (2023)

The increasing number of anti-cancer drugs poses a challenge for doctors who need to choose the best treatment for patients. Precision cancer medicine aims to match a drug with a tumors unique characteristics to maximize its effectiveness. However, current approaches don’t fully consider tumor heterogeneity or differences within the cells that make up the tumor,…

Nanopore Sequencing of Cell-Free DNA as a Biomarker for ALL, Frontiers in Oncology (2022)

Acute Lymphoblastic Leukemia (ALL) is a common type of cancer primarily affecting children. Unfortunately, patients who experience a relapse of ALL tend to have a poor prognosis. Detecting any remaining cancer cells, called minimal residual disease (MRD), and identifying its spread to the central nervous system (CNS), are crucial factors for predicting the disease’s outcome.…

Al-Hamaly MA, Cox AH, Haney MG, Zhang W, Arvin EC, Sampathi S, Wimsett M, Liu C, Blackburn JS. Zebrafish drug screening identifies Erlotinib as an inhibitor of Wnt/β-catenin signaling and self-renewal in T-cell acute lymphoblastic leukemia. Biomedicine & Pharmacotherapy. 2024 Jan;170:116013. doi: 10.1016/j.biopha.2023.116013. Epub 2023 Dec 16. PMID: 38104416; PMCID: PMC10833092. [Full Text]

Jolly JT, Cheatham TC, Blackburn JS. Phosphatase and Pseudo-Phosphatase Functions of Phosphatase of Regenerating Liver 3 (PRL-3) Are Insensitive to Divalent Metals In Vitro. ACS Omega. 2023 Aug 9;8(33):30578-30589. doi: 10.1021/acsomega.3c04095. PMID: 37636930; PMCID: PMC10448674. [Full Text]

Mitchell RJ, Gowda AS, Olivelli AG, Huckaba AJ, Parkin S, Unrine JM, Oza V, Blackburn JS, Ladipo F, Heidary DK, Glazer EC. Triarylphosphine-Coordinated Bipyridyl Ru(II) Complexes Induce Mitochondrial Dysfunction. Inorg Chem. 2023 Jul 17;62(28):10940-10954. doi: 10.1021/acs.inorgchem.3c00736. PMID: 37405779. [Full Text]

Smith CN, Kihn K, Williamson ZA, Chow KM, Hersh LB, Korotkov KV, Deredge D, Blackburn JS. Development and characterization of nanobodies that specifically target the oncogenic Phosphatase of Regenerating Liver-3 (PRL-3) and impact its interaction with a known binding partner, CNNM3. PLoS One. 2023 May 23;18(5):e0285964. doi: 10.1371/journal.pone.0285964. PMID: 37220097; PMCID: PMC10204944. [Full Text]

Mitchell RJ, Kriger SM, Fenton AD, Havrylyuk D, Pandeya A, Sun Y, Smith T, DeRouchey JE, Unrine JM, Oza V, Blackburn JS, Wei Y, Heidary DK, Glazer EC. A monoadduct generating Ru(ii) complex induces ribosome biogenesis stress and is a molecular mimic of phenanthriplatin. RSC Chem Biol. 2023 Feb 27;4(5):344-353. doi: 10.1039/d2cb00247g. PMID: 37181632; PMCID: PMC10170627. [Full Text]

Al-Hamaly MA, Turner LT, Rivera-Martinez A, Rodriguez A, Blackburn JS. Zebrafish Cancer Avatars: A Translational Platform for Analyzing Tumor Heterogeneity and Predicting Patient Outcomes. Int J Mol Sci. 2023 Jan 24;24(3):2288. doi: 10.3390/ijms24032288. PMID: 36768609; PMCID: PMC9916713. [Full Text]

Sampathi S, Chernyavskaya Y, Haney MG, Moore LH, Snyder IA, Cox AH, Fuller BL, Taylor TJ, Yan D, Badgett TC, Blackburn JS. Nanopore sequencing of clonal IGH rearrangements in cell-free DNA as a biomarker for acute lymphoblastic leukemia. Front Oncol. 2022 Dec 14;12:958673. doi: 10.3389/fonc.2022.958673. PMID: 36591474; PMCID: PMC9795051. [Full Text]

Chernyavskaya Y, Zhang X, Liu J, Blackburn J. Long-read sequencing of the zebrafish genome reorganizes genomic architecture. BMC Genomics. 2022 Feb 10;23(1):116. doi: 10.1186/s12864-022-08349-3. PMID: 35144548; PMCID: PMC8832730. [Full Text]

Ryan RT, Havrylyuk D, Stevens KC, Moore LH, Parkin S, Blackburn JS, Heidary DK, Selegue JP, Glazer EC. Biological Investigations of Ru(II) Complexes With Diverse β-diketone Ligands. Eur J Inorg Chem. 2021 Sep 21;2021(35):3611-3621. doi: 10.1002/ejic.202100468. Epub 2021 Jul 21. PMID: 34539235; PMCID: PMC8447810. [Full Text]

Smith, C.N. and Blackburn, J.S. (2021), PRL-3 promotes a positive feedback loop between STAT1/2-induced gene expression and glycolysis in multiple myeloma. FEBS J. https://doi.org/10.1111/febs.16120 [Full Text]

Rivas DR, Dela Cerna MVC, Smith CN, Sampathi S, Patty BG, Lee D, Blackburn JS. A screen of FDA-approved drugs identifies inhibitors of protein tyrosine phosphatase 4A3 (PTP4A3 or PRL-3). Sci Rep. 2021 May 13;11(1):10302. doi: 10.1038/s41598-021-89668-5. [Full Text]

Haney MG, Wimsett M, Liu C, Blackburn JS. Protocol for rapid assessment of the efficacy of novel Wnt inhibitors using zebrafish models. STAR Protoc. 2021 Apr 1;2(2):100433. doi: 10.1016/j.xpro.2021.100433. [PMC Link]

Zhang W, Sviripa VM, Xie Y, Yu T, Haney MG, Blackburn JS, Adeniran CA, Zhan CG, Watt DS, Liu C. Epigenetic Regulation of Wnt Signaling by Carboxamide-Substituted Benzhydryl Amines that Function as Histone Demethylase Inhibitors. iScience. 2020 Nov 13;23(12):101795. doi: 10.1016/j.isci.2020.101795. [Full Text]

Garcia EG, Veloso A, Oliveira ML, Allen JR, Loontiens S, Brunson D, Do D, Yan C, Morris R, Iyer S, Garcia SP, Iftimia N, Van Loocke W, Matthijssens F, McCarthy K, Barata JT, Speleman F, Taghon T, Gutierrez A, Van Vlierberghe P, Haas W, Blackburn JS, Langenau DM. PRL3 enhances T-cell acute lymphoblastic leukemia growth through suppressing T-cell signaling pathways and apoptosis. Leukemia. 2021 Mar;35(3):679-690. doi: 10.1038/s41375-020-0937-3. Epub 2020 Jun 30. [Full Text]

Haney MG, Moore LH, Blackburn JS. Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish. J Vis Exp. 2020 Apr 10;(158). doi: 10.3791/60996. [PubMed Link]

Wei M, Haney MG, Rivas DR, Blackburn JS. Protein tyrosine phosphatase 4A3 (PTP4A3/PRL-3) drives migration and progression of T-cell acute lymphoblastic leukemia in vitro and in vivo. Oncogenesis. 2020, 9(1):6. [PubMed Link] [Full Text]

Xie Y, Kril LM, Zhang W, Bondarenko SP, Kondratyuk KM, Hausman ES, Martin ZM, Wyrebek PP, Liu X, Deacuic A, Dowskin LP, Chen J, Zhu H, Zhan CG, Sviripa VM, Blackburn J, Watt DS, Liu C. Semisynthetic aurones inhibit tubulin polymerization at the colchicine-binding site and repress PC-3 tumor xenografts in nude mice and myc-induced T-ALL in zebrafish. Science Reports. 2019, 9(1):6439. [PubMed Link] [Full Text]

Wei M, Korotkov KV, Blackburn JS. Targeting phosphatases of regenerating liver (PRLs) in cancer. Pharmacology and Therapeutics. 2018; Oct(190) 128-138. [PubMed Link] [Full Text]

Lobbardi R, Pinder J, Martinze-Pastor B, Blackburn JS, Abraham B, Langenau DM. Tox is an oncogeneic driver in T-cell Acute Lymphoblastic Leukemia. Cancer Discovery. 2017; 7(11) 1336-1353. [PubMed Link] [Full Text]

Moore, F.E.;Garcia, E.G.;Lobbardi, R.;Jain, E.;Tang, Q.;Moore, J.C.;Cortes, M.;Molodtsov, A.;Kasheta, M.;Luo, C.C.;Garcia, A.J.;Mylvaganam, R.;Yoder, J.A.;Blackburn, J.S.;Sadreyev, R.I.;Ceol, C.J.;North, T.E.;Langenau, D.M. “Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.” The Journal of experimental medicine 213, 6 (2016): 979-92. [PubMed Link] | [Full Text]

Tang, Q.;Moore, J.C.;Ignatius, M.S.;Tenente, I.M.;Hayes, M.N.;Garcia, E.G.;Torres Yordán, N.;Bourque, C.;He, S.;Blackburn, J.S.;Look, A.T.;Houvras, Y.;Langenau, D.M. “Imaging tumour cell heterogeneity following cell transplantation into optically clear immune-deficient zebrafish.” Nature communications 7,(2016): 10358. [PubMed Link] | [Full Text]

Blackburn, J.S.;Liu, S.;Wilder, J.L.;Dobrinski, K.P.;Lobbardi, R.;Moore, F.E.;Martinez, S.A.;Chen, E.Y.;Lee, C.;Langenau, D.M. “Clonal evolution enhances leukemia-propagating cell frequency in T cell acute lymphoblastic leukemia through Akt/mTORC1 pathway activation.” Cancer cell 25, 3 (2014): 366-78. [PubMed Link] | [Full Text]

Blackburn, J.S.;Langenau, D.M. “Zebrafish as a model to assess cancer heterogeneity, progression and relapse.” Disease models & mechanisms 7, 7 (2014): 755-62. [PubMed Link] | [Full Text]

Tang, Q.;Abdelfattah, N.S.;Blackburn, J.S.;Moore, J.C.;Martinez, S.A.;Moore, F.E.;Lobbardi, R.;Tenente, I.M.;Ignatius, M.S.;Berman, J.N.;Liwski, R.S.;Houvras, Y.;Langenau, D.M. “Optimized cell transplantation using adult rag2 mutant zebrafish.” Nature methods 11, 8 (2014): 821-4. [PubMed Link] | [Full Text]

Blackburn, J.S.;Liu, S.;Raiser, D.M.;Martinez, S.A.;Feng, H.;Meeker, N.D.;Gentry, J.;Neuberg, D.;Look, A.T.;Ramaswamy, S.;Bernards, A.;Trede, N.S.;Langenau, D.M. “Notch signaling expands a pre-malignant pool of T-cell acute lymphoblastic leukemia clones without affecting leukemia-propagating cell frequency.” Leukemia 26, 9 (2012): 2069-78. [PubMed Link] | [Full Text]

Ignatius, M.S.;Chen, E.;Elpek, N.M.;Fuller, A.Z.;Tenente, I.M.;Clagg, R.;Liu, S.;Blackburn, J.S.;Linardic, C.M.;Rosenberg, A.E.;Nielsen, P.G.;Mempel, T.R.;Langenau, D.M. “In vivo imaging of tumor-propagating cells, regional tumor heterogeneity, and dynamic cell movements in embryonal rhabdomyosarcoma.”Cancer cell 21, 5 (2012): 680-93. [PubMed Link] | [Full Text]

Zheng, S.;Ghitani, N.;Blackburn, J.S.;Liu, J.P.;Zeitlin, S.O. “A series of N-terminal epitope tagged Hdh knock-in alleles expressing normal and mutant huntingtin: their application to understanding the effect of increasing the length of normal Huntingtin’s polyglutamine stretch on CAG140 mouse model pathogenesis.” Molecular brain 5, (2012): 28. [PubMed Link] | [Full Text]

Moore, F.E.;Reyon, D.;Sander, J.D.;Martinez, S.A.;Blackburn, J.S.;Khayter, C.;Ramirez, C.L.;Joung, J.K.;Langenau, D.M. “Improved somatic mutagenesis in zebrafish using transcription activator-like effector nucleases (TALENs).” PloS one 7, 5 (2012): e37877. [PubMed Link] | [Full Text]

Blackburn, J.S.;Liu, S.;Langenau, D.M. “Quantifying the frequency of tumor-propagating cells using limiting dilution cell transplantation in syngeneic zebrafish.” Journal of visualized experiments : JoVE 53(2011): e2790. [PubMed Link] | [Full Text]

Sander, J.D.;Dahlborg, E.J.;Goodwin, M.J.;Cade, L.;Zhang, F.;Cifuentes, D.;Curtin, S.J.;Blackburn, J.S.;Thibodeau-Beganny, S.;Qi, Y.;Pierick, C.J.;Hoffman, E.;Maeder, M.L.;Khayter, C.;Reyon, D.;Dobbs, D.;Langenau, D.M.;Stupar, R.M.;Giraldez, A.J.;Voytas, D.F.;Peterson, R.T.;Yeh, J.R.;Joung, J.K.“Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA).” Nature methods 8, 1 (2011): 67-9. [PubMed Link] | [Full Text]

Blackburn, J.S.;Liu, S.;Raimondi, A.R.;Ignatius, M.S.;Salthouse, C.D.;Langenau, D.M. “High-throughput imaging of adult fluorescent zebrafish with an LED fluorescence macroscope.” Nature protocols 6, 2(2011): 229-41. [PubMed Link] | [Full Text]

Blackburn, J.S.;Langenau, D.M. “aMAZe-ing tools for mosaic analysis in zebrafish.” Nature methods 7, 3(2010): 188-90. [PubMed Link] | [Full Text]

Smith, A.C.;Raimondi, A.R.;Salthouse, C.D.;Ignatius, M.S.;Blackburn, J.S.;Mizgirev, I.V.;Storer, N.Y.;Jong, J.L.;Chen, A.T.;Zhou, Y.;Revskoy, S.;Zon, L.I.;Langenau, D.M. “High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia.” Blood 115, 16 (2010): 3296-303. [PubMed Link] | [Full Text]

Eck, S.M.;Blackburn, J.S.;Schmucker, A.C.;Burrage, P.S.;Brinckerhoff, C.E. “Matrix metalloproteinase and G protein coupled receptors: co-conspirators in the pathogenesis of autoimmune disease and cancer.” Journal of autoimmunity 33, 3-4 (2009): 214-21. [PubMed Link] | [Full Text]

Blackburn, J.S.;Brinckerhoff, C.E. “Wild-type versus mutant MMP-8 in melanoma: ‘when you come to a fork in the road, take it’.” Pigment cell & melanoma research 22, 3 (2009): 248-50. [PubMed Link] | [Full Text]

Blackburn, J.S.;Liu, I.;Coon, C.I.;Brinckerhoff, C.E. “A matrix metalloproteinase-1/protease activated receptor-1 signaling axis promotes melanoma invasion and metastasis.” Oncogene 28, 48 (2009): 4237-48. [PubMed Link] | [Full Text]

Blackburn, J.S.;Brinckerhoff, C.E. “Matrix metalloproteinase-1 and thrombin differentially activate gene expression in endothelial cells via PAR-1 and promote angiogenesis.” The American journal of pathology173, 6 (2008): 1736-46. [PubMed Link] | [Full Text]

Blackburn, J.S.;Rhodes, C.H.;Coon, C.I.;Brinckerhoff, C.E. “RNA interference inhibition of matrix metalloproteinase-1 prevents melanoma metastasis by reducing tumor collagenase activity and angiogenesis.” Cancer research 67, 22 (2007): 10849-58. [PubMed Link] | [Full Text]